Hif1α antibody
WebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … WebAnti-HIF-1α Antibody is a Rabbit Polyclonal Antibody for detection of HIF-1 alpha also known as ARNT interacting protein or HIF-1 alpha & has been validated in WB. - Find MSDS or SDS, a COA, data sheets and more information.
Hif1α antibody
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WebHIF-1α Antibody (C-19) has been replaced by a more specific monoclonal antibody, HIF-1α (28b) that provides a stronger signal & more reproducible data. Choose a Store Santa cruz biotechnology. Santa Cruz Animal Health. Choose a Language ... WebWestern blot - HIF1α Rabbit pAb (A11945) Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
WebView Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC + more. Species Reactivity: Human, Mouse, Rat + … WebImmunoprecipitation of HIF-1α from lysate of Hep G2 cells treated with cobalt chloride (100 µM, 4 h). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is HIF-1α (D1S7W) XP ® Rabbit mAb. Western blot analysis was performed using HIF-1α (D1S7W) XP ® Rabbit mAb. Anti-rabbit IgG, HRP-linked …
WebAnti-HIF-1α Antibody is a Rabbit Polyclonal Antibody for detection of HIF-1 alpha also known as ARNT interacting protein or HIF-1 alpha & has been validated in WB. … Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional …
Web8 de abr. de 2024 · The percentage of positive tumour cells was scored for each antibody, using only nuclear staining for HIF-1α, and only membranous staining for CA-IX. Cut-off values of the percentages for dichotomisation of the data were in accordance with the cut-off values used in previous studies performed in our institution [ 13 , 14 ], and were 0.5% for …
Web11 de abr. de 2024 · The following antibodies were used: p53, PHD2, MMP2, HIF-1α, MRP1, and GAPDH, and the secondary antibody (detailed in Supplementary Table S1). Quantitative RT-PCR: In order to further compare the mRNA transcription levels of 3d-ACS and 2d cultured GC cells, the total RNA in the released cells was extracted using Trizol … opening prayer for life groupWeb27 de dez. de 2024 · HIF-1α is involved in blood-brain barrier dysfunction and paracellular migration of bacteria in ... This antibody reacts with human, mouse, rat, bovine, canine, guinea pig, primate, xenopus, zebrafish. The HIF-1 alpha Antibody - BSA Free has been validated for the following applications: Western Blot, Simple Western, ELISA ... opening prayer for laity sundayWebAnti-HIF-1 alpha antibody [EPR16897] (ab179483) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … opening prayer for leadership meetingWebMonoclonal Antibody for studying HIF1A. Cited in 220 publications. Validated for WB, ChIP, ChIP, C&R. Highly specific and rigorously … opening prayer for maundy thursdayWeb18 de mar. de 2024 · Membranes were blocked with 5% milk powder in 1× PBS-T for 1 h and then incubated overnight at 4 °C with a mouse anti-HIF-1α monoclonal antibody in 1% milk powder of PBS-T buffer. After overnight incubation, the membranes were washed in 1× PBS-T three times for 5 min each, and were incubated for 1 h in the goat polyclonal anti … i own ape” nftWebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α (D5F3M) Mouse Monoclonal Antibody (CST #79233) is ready to ship. opening prayer for mothering sundayWeb23 de jul. de 2024 · HIF-1α Antibody (28b) is an IgG 1 κ mouse monoclonal HIF-1 alpha antibody (also designated HIF1A antibody or HIF-1 alpha antibody) suitable for the … opening prayer for meeting group