Phosphinothricin acetyltransferase gene
WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebDownload scientific diagram Schematic structure of the T-DNA region of the binary vector pCAMBIA3301. LB, left border; RB, right border; bar, phosphinothricin acetyltransferase gene; gus-int, β ...
Phosphinothricin acetyltransferase gene
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WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said …
WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of …
WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar WebApr 23, 2012 · The phosphinothricin expression cassette consists of the NOS promoter (promoter of the A. tumefaciens nopaline synthase gene), the pat cds (phosphinothricin acetyltransferase gene from Streptomyces viridochromogenes), and the NOS terminator (terminator of the A. tumefaciens nopaline synthase gene).
WebThe gene encodes phosphinothricin acetyltransferase, which N-acetylates phosphinothricin using acetyl-CoA. Figure 6.1 . Overview of biosynthetic pathways of a number of …
WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... soil thermometers for saleWebCloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli Gene 63 65-74 1988 Streptomyces viridochromogenes, Streptomyces viridochromogenes Tue494 3290054. brenda. 673843. sludge feed pumpWebMar 18, 2016 · Gene: BASTA selectable marker from Streptomyces hygrosopicus- phosphinothricin acetyltransferase gene to confer BASTA resistance as a selectable marker Promoter: 35S promoter from Cauliflower mosaic caulimovirus – CaMV 35S constitutive promoter to drive expression of GUS reporter gene soil thermometer tractor supplyWebThis gene encodes neomycin phosphotransferase, which confers resistance to antibiotics such as kanamycin, gentamicin A, neomycin, and others when expressed in plants. The hpt gene, also isolated from E. coli, codes for hygromycin transferase and confers resistance … soil thermometers reviewsWebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. sludge fen classicWebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … sludgefishingWebIn this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. ... GmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) ... soil thermometer ppt